Dosage guide · 20 mg vial

Vilon Dosage Protocol

Lys-Glu · synthetic dipeptide peptide bioregulator

Vilon's reference protocol starts at 67 mcg on day 1 and increases daily to 333 mcg on day 5; later courses use 333–667 mcg for five days every four weeks. With 6 mL in a 20 mg vial, the concentration is 3.33 mg/mL and 67 mcg is 2.01 units on a U-100 syringe.

How often5 days every 4 weeks

Starting dose67 mcg

Start = units2 units

Mix6 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

Vilon 20 mg vial

Not currently stocked by Peak Peptides Australia

No product photo — this strength isn't stocked by Peak

Dosage chart How to mix Supplies Calculator About Vilon References

01 · DOSAGE CHART

Vilon dosage chart

Five-day monthly approach · 20 mg vial + 6 mL BAC water

BAC water added

Concentration3.33 mg/mL

1 unit (U-100)33.3 mcg= 0.01 mL

How often5 days every 4 weeksSubcutaneous injection

The reference uses 3 mL; 6 mL is the default so the 67 mcg starting amount measures 2.01 U-100 units, subject to vial capacity.

Vilon dosage chart for a 20 mg vial mixed with 6 mL of bacteriostatic water
Days	Dose each time	Syringe units
Day 1, first courseStart	67 mcg	2 units0.02 mL
Day 2, first course	133 mcg	4 units0.04 mL
Day 3, first course	200 mcg	6 units0.06 mL
Day 4, first course	267 mcg	8 units0.08 mL
Day 5, first course	333 mcg	10 units0.1 mL
Later courses, days 1–5Maintenance	333–667 mcg	10–20 units0.1–0.2 mL

Once daily for 5 days, then 23 days off · subcutaneous injection

Use the five daily amounts in order during the first course, then no doses for the remaining 23 days of the four-week cycle.

Later courses use 333–667 mcg once daily for five consecutive days; the source does not specify how to choose within this range.

This is an educational reference schedule, not a clinically established human dose.

Community-reported reference amounts — Vilon has no approved or clinically established human dose in Australia. Education only, not medical advice. Dose amounts adapted from Vilon 20 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 20 mg vial with 6 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 6 full draws of bacteriostatic water gives 3.33 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 6 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 3.33 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 20 mg vial mixed with 6 mL.

Length of protocol

1Vilon 20 mg vial4.33 mg needed in total

111 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle6 mL used for mixing

22Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume6 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

3.33 mg/mL

(3333 mcg/mL)

Calculated Liquid Volume

0.020 mL

Equivalent U-100 volume scale position

2markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About Vilon

Protocol overview

Starting amount: 67 mcg on day 1 of the first course
How it increases: 133, 200, 267 and 333 mcg on days 2–5
Later courses: 333–667 mcg daily for five days
Frequency: Five consecutive days every four weeks
Course length: 2–4 four-week courses in the reference protocol
Default mix: 6 mL bacteriostatic water → 3.33 mg/mL

Storage

Before mixing: Follow the exact vial label; keep the dry vial protected from light.
After mixing: The reference protocol says refrigerate at 2–8 °C and protect from light; follow the product-specific use-by period.
Never: Do not assume freezing extends stability, repeatedly freeze and thaw, or use a cloudy vial with particles.

How it works

Vilon is the synthetic dipeptide Lys-Glu, also called lysylglutamic acid. An FDA substance record lists Vilon dihydrate as a synonym of lysylglutamic acid dihydrate.

In laboratory work, Lys-Glu increased interleukin-2 gene expression in mouse spleen lymphocytes. Cultured human and rat thymus cells exposed to Vilon-related dipeptides showed changes in CD5 and T-cell differentiation markers; these are cell-culture findings, not evidence of an immune benefit in people.

A 2022 experiment treated THP-1 monocytes and macrophages with Vilon among several peptides. The observations on inflammatory signalling and cell adhesion were in vitro, so they do not establish an effect after injection in people.

Illustrated mechanism explainer

What the research shows

The FDA substance record identifies Vilon dihydrate as lysylglutamic acid dihydrate; the parent active moiety has molecular weight 275.3.
In vitro, Lys-Glu stimulated interleukin-2 gene expression in mouse spleen lymphocytes, with the effect depending on concentration and treatment duration.
A 2013 experiment in cultured human and rat thymus cells reported increased CD5 expression and differentiated T-cell markers. It was a cell-culture experiment, not a clinical dosing study.
A 2022 THP-1 cell experiment used 100 ng/mL of each peptide, including Vilon, and assessed cells after overnight treatment or 2, 4, 8 and 12 hours. These laboratory results cannot establish human benefit or safety.
In aged rats, oral Vilon given for 1 month changed glucose transport measures in the small intestine. This does not establish a dose or outcome in people.
No Vilon drug-package label was returned by DailyMed, and the Australian ARTG search did not identify a Vilon therapeutic-good entry. Vilon is not an approved medicine in Australia.

Reported side effects

Published sources checked here do not provide a reliable human adverse-event rate or an established safety profile for subcutaneous Vilon.
The 2022 Vilon findings were from cells treated in vitro at 100 ng/mL, so they cannot predict adverse effects after use in people.
DailyMed returned 0 Vilon drug-package labels, so there is no US label listing contraindications, interactions or adverse effects for this product.
Do not treat the 1-month oral rat experiment or cultured-cell findings as injection safety data.

Important notes

Vilon is not an approved medicine in Australia. The ARTG is the TGA database for therapeutic goods that can be supplied in Australia; the Vilon keyword search did not identify a matching Vilon therapeutic-good entry.
WADA's 2026 Prohibited List does not name Vilon or Lys-Glu. Its S0 category prohibits pharmacological substances with no current approval by any governmental regulatory health authority; athletes should obtain an eligibility decision from their anti-doping organisation before use.
There is no clinically established human dose or adequate subcutaneous safety dataset. The chart is copied from an educational reference protocol, not a clinical label.
Vilon is not a cancer treatment. A 2005 report describes preliminary combined-treatment experience in elderly colorectal-cancer patients but its abstract does not report the Vilon dose, participant count or adverse-event rates.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

Vilon questions

How many U-100 units is 67 mcg of Vilon?

With 6 mL in a 20 mg vial, the concentration is 3.33 mg/mL. A 67 mcg amount is 2.01 U-100 units, or 0.0201 mL.

How much bacteriostatic water is used for Vilon 20 mg?

The reference protocol uses 3 mL, which makes 6.67 mg/mL. This page defaults to 6 mL, which makes 3.33 mg/mL and keeps the 67 mcg start amount at 2.01 U-100 units; confirm the vial can hold this volume.

How much Vilon is used in the first five-day course?

The first five amounts total 1,000 mcg, or 1 mg. At the 6 mL default mix, that total is 30 U-100 units, or 0.30 mL, across the five injections.

How many units is 333 mcg or 667 mcg of Vilon?

At the 6 mL default mix, 333 mcg is 9.99 U-100 units, or 0.0999 mL. A 667 mcg amount is 20.01 units, or 0.2001 mL.

07 · SOURCES