Dosage guide · 20 mg vial

Pinealon Dosage Protocol

Glu-Asp-Arg (EDR) · synthetic tripeptide bioregulator

The reference protocol starts at 1 mg daily for days 1–5, then 1.5 mg for days 6–14 and 2 mg for days 15–20. A 20 mg vial plus 3 mL gives 6.67 mg/mL, so the 1 mg start is 15 units (0.15 mL) on a U-100 syringe.

How oftenOnce daily

Starting dose1 mg

Start = units15 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

Pinealon 20 mg vial

Not currently stocked by Peak Peptides Australia

No product photo — this strength isn't stocked by Peak

Dosage chart How to mix Supplies Calculator About Pinealon References

01 · DOSAGE CHART

Pinealon dosage chart

20-day daily approach · 20 mg vial + 3 mL BAC water

BAC water added

Concentration6.67 mg/mL

1 unit (U-100)66.7 mcg= 0.01 mL

How oftenOnce dailySubcutaneous injection

3 mL is the mix used by the attributed 20 mg reference protocol, giving 6.67 mg/mL.

Pinealon dosage chart for a 20 mg vial mixed with 3 mL of bacteriostatic water
Days	Daily dose	Syringe units
Days 1–5Start	1 mg	15 units0.15 mL
Days 6–14	1.5 mg	22.5 units0.225 mL
Days 15–20Maintenance	2 mg	30 units0.3 mL

Once daily · morning or early afternoon · subcutaneous injection

The attributed reference describes 10–20 day cycles and an optional extension to 28 days.

At the same reference, repeat cycles are spaced 2–3 months apart.

A full 20-day stepped schedule totals 30.5 mg, so it needs two 20 mg vials.

Reference-site educational amounts — Pinealon has no clinically established human dose in Australia or the United States. Education only, not medical advice. Dose amounts adapted from Pinealon 20 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 20 mg vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 6.67 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 6.67 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 20 mg vial mixed with 3 mL.

Length of protocol

1Pinealon 20 mg vial18.5 mg needed in total

151 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle3 mL used for mixing

30Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

Concentration

6.67 mg/mL

(6667 mcg/mL)

Calculated Liquid Volume

0.150 mL

Equivalent U-100 volume scale position

15markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About Pinealon

Protocol overview

Starting dose: 1 mg once daily for days 1–5
How it increases: 1.5 mg on days 6–14, then 2 mg on days 15–20
Frequency: Once daily
When to take it: Morning or early afternoon in the reference protocol
Cycle length: 10–20 days; the reference notes an optional 28-day extension
Common mix: 3 mL bacteriostatic water → 6.67 mg/mL

Storage

Before mixing: The cited reference protocol says to keep the lyophilised vial frozen at −20 °C in dry, dark conditions.
After mixing: The cited reference protocol says to refrigerate at 2–8 °C and protect from light.
Never: Avoid repeated freeze–thaw cycles.

How it works

Pinealon, also called EDR, is a synthetic tripeptide with the sequence Glu-Asp-Arg.

Cell and rodent research has explored whether EDR affects oxidative-stress responses, cell survival and gene expression. These proposed mechanisms have not established a clinical benefit or a human dose.

Illustrated mechanism explainer

What the research shows

A 2011 cell and animal paper reported dose-dependent reductions in oxidative-stress-related reactive oxygen species and necrotic cell death in rat cerebellar granule cells, neutrophils and PC12 cells. This is preclinical evidence, not a human outcome.
In a rat prenatal-hyperhomocysteinaemia model, pregnant rats received Pinealon 10 micrograms/kg/day intraperitoneally for 5 days before methionine loading. The 45-day behavioural experiment used 23 offspring in each group; this does not establish a human dose.
A 2015 PubMed-indexed abstract combined Pinealon with Vesugen in a reported 32 people aged 41–83. It did not provide a Pinealon-only dose, randomisation details or an adverse-event rate, and its reported sex counts total 30 rather than 32.
A ClinicalTrials.gov query for Pinealon returned no study records when checked for this guide. A current DailyMed query also returned no US drug-label records for Pinealon.
No Australian ARTG entry or US FDA label was identified in the searches for this guide; the chart is not a TGA or US FDA approved dosing schedule.

Reported side effects

Adequate controlled human safety data and a Pinealon-only adverse-event rate were not identified. The small 2015 report combined Pinealon with Vesugen, so its findings cannot be assigned to Pinealon alone.
The 2015 abstract reported prooxidant activity and a decrease in CD34-positive haematopoietic cells in its combined-peptide group; the clinical meaning and causation are uncertain.
The TGA warns that unapproved injectable peptides may be poorly labelled and that identity, strength, sterility, contaminants and toxins may be unknown.

Important notes

These are reference-site educational amounts, not a TGA- or US FDA-approved Pinealon dose regimen.
Human evidence is sparse and does not establish Pinealon as a treatment for sleep, cognitive problems, neurodegeneration or any other condition.
For athletes, Pinealon is not named in the 2026 WADA List. WADA S0 prohibits pharmacological substances with no current approval by a governmental health authority, so check directly with an anti-doping organisation before use.
The TGA advises caution with unapproved peptide products because vial identity, concentration, sterility and contaminants may be uncertain.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

Pinealon questions

How many units is 1 mg of Pinealon in a 20 mg vial?

With 3 mL in a 20 mg vial, the concentration is 6.67 mg/mL. A 1 mg dose is 15 units, or 0.15 mL, on a U-100 syringe. At the same mix, 1.5 mg is 22.5 units (0.225 mL) and 2 mg is 30 units (0.30 mL).

How much bacteriostatic water is used for 20 mg Pinealon?

The attributed 20 mg reference protocol uses 3 mL of bacteriostatic water. That produces 6.67 mg/mL, where 15 units equals 1 mg and 30 units equals 2 mg on a U-100 syringe.

How long does a 20 mg Pinealon vial last?

At 1 mg once daily, 20 mg lasts 20 days. At 1.5 mg once daily it lasts about 13.3 days, and at 2 mg once daily it lasts 10 days. The complete 20-day stepped reference schedule totals 30.5 mg, so it requires two 20 mg vials.

07 · SOURCES