Reconstitution Guide
A step-by-step guide on how to properly mix lyophilized peptides with bacteriostatic water for research purposes.
1Gather Supplies
Bacteriostatic Water
Sterile water containing 0.9% benzyl alcohol to prevent bacterial growth.
Alcohol Swabs
To sterilize vial stoppers and injection sites before use.
2Mixing Process
Clean the Vials
Remove the plastic caps from both the peptide vial and the bacteriostatic water vial. Wipe the rubber stoppers of both vials with an alcohol swab. Allow them to dry for a few seconds.
Draw the Water
Using a sterile syringe, draw the desired amount of bacteriostatic water (typically 1mL, 2mL, or 3mL depending on the protocol). Ensure there are no large air bubbles in the syringe.
Inject into Peptide Vial
Insert the needle into the center of the peptide vial's rubber stopper. Important: Aim the needle toward the glass wall of the vial, not directly onto the powder. Inject the water slowly, allowing it to trickle down the side of the glass.
Dissolve Gently
Once all water is added, remove the syringe. DO NOT SHAKE THE VIAL.Gently swirl or roll the vial between your fingers until all the powder is dissolved. The solution should be clear.
3Storage
Refrigeration is Key
Once reconstituted, peptides must be stored in the refrigerator at 2–8°C (36–46°F).
Shelf Life
Most reconstituted peptides remain stable for 4-8 weeks when stored properly.
Avoid Freezing
Do not freeze peptides after they have been mixed with water, as this can damage the molecular structure.