Dosage guide · 10 mg vial

DSIP Dosage Protocol

Delta sleep-inducing peptide · emideltide nonapeptide

The reference protocol lists 100 mcg nightly in week 1, then 150 mcg in week 2, 200 mcg in week 3 and 250–300 mcg for weeks 4–8. With 3 mL of bacteriostatic water, a 10 mg vial is 3.33 mg/mL, so the 100 mcg starting amount is 3 units on a U-100 syringe.

How oftenOnce daily

Starting dose100 mcg

Start = units3 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

DSIP 10 mg vial

View on Peak Peptides Also at Pure Performance

Exact Peak Peptides Australia product photo

Dosage chart How to mix Supplies Calculator About DSIP References

01 · DOSAGE CHART

DSIP dosage chart

Gradual nightly approach · 10 mg vial + 3 mL BAC water

BAC water added

Concentration3.33 mg/mL

1 unit (U-100)33.3 mcg= 0.01 mL

How oftenOnce dailySubcutaneous injection

3 mL is the mix shown on Peak's DSIP page and the reference protocol; Pure's page instead lists 2 mL.

DSIP dosage chart for a 10 mg vial mixed with 3 mL of bacteriostatic water
Week	Daily dose	Syringe units
Week 1Start	100 mcg	3 units0.03 mL
Week 2	150 mcg	4.5 units0.045 mL
Week 3	200 mcg	6 units0.06 mL
Weeks 4–8Maintenance	250–300 mcg	7.5–9 units0.075–0.09 mL

Once daily · evening · subcutaneous injection

The reference chart places the once-daily amount in the evening, about 30–60 minutes before bedtime.

No amount beyond week 8 is shown in the reference protocol.

The reference site describes this as a community-reported measurement schedule, not a clinically validated regimen.

Community-reported amounts — DSIP has no approved or clinically established subcutaneous dose. Education only, not medical advice. Dose amounts adapted from DSIP 10 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 10 mg vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 3.33 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 3.33 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 10 mg vial mixed with 3 mL.

Length of protocol

1DSIP 10 mg vial5.25 mg needed in total

291 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle3 mL used for mixing

58Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

DSIP 10 mg vialPeak Peptides Australia DSIP 10 mg vialPure Performance Australia

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

3.33 mg/mL

(3333 mcg/mL)

Calculated Liquid Volume

0.030 mL

Equivalent U-100 volume scale position

3markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About DSIP

Protocol overview

Starting dose: 100 mcg once nightly in week 1
How it increases: 150 mcg in week 2, 200 mcg in week 3, then 250–300 mcg in weeks 4–8
Frequency: Once daily
When to take it: Evening, about 30–60 minutes before bedtime in the reference protocol
Protocol span: 8 weeks shown by the reference protocol
Common mix: 3 mL bacteriostatic water → 3.33 mg/mL

Storage

Before mixing: Store the dry lyophilised compound frozen at −20 °C, dry, dark and protected from light.
After mixing: Refrigerate at 2–8 °C and protect from light.
Never: Avoid repeated freeze–thaw cycles or using a solution that is cloudy or has particles.

How it works

DSIP, also called emideltide, is a nine-amino-acid peptide historically investigated in sleep physiology and neuroendocrine research. Its name reflects early animal work rather than an established treatment effect.

The proposed biology remains unresolved. A 2006 review found that the DSIP gene, precursor protein and possible related receptor had not been isolated, and described the sleep-factor hypothesis as weakly documented.

Illustrated mechanism explainer

What the research shows

In a 1981 double-blind crossover experiment, six healthy volunteers received slow intravenous DSIP at 25 nmol/kg; median total sleep time during a 130-minute observation interval was 59% higher than with placebo. This was intravenous research, not the subcutaneous schedule shown above.
A 1992 double-blind study in 16 people with chronic insomnia used intravenous DSIP at 25 nmol/kg before three nights. Objective sleep efficiency was higher and sleep latency shorter than placebo, but the authors judged the effects weak and found no change in subjective sleep quality.
FDA's 2026 assessment found no data supporting emideltide effectiveness for chronic insomnia, narcolepsy or opioid withdrawal by the proposed subcutaneous route, and no clinical safety data for that route.
Neither emideltide free base nor emideltide acetate is a component of an FDA-approved drug. Searches of the public ARTG for DSIP, emideltide and delta sleep-inducing peptide returned no matching record when checked on 25 October 2026.
A ClinicalTrials.gov intervention search for delta sleep-inducing peptide, DSIP or emideltide returned no records when checked on 25 October 2026.

Reported side effects

There are no clinical safety data for the proposed subcutaneous route in FDA's emideltide assessment, so its risks for that route are not established.
FDA reported transient headache, nausea and vertigo as common side effects in older intravenous withdrawal reports, with cases of progressive hypotension after the second intravenous injection; withdrawal symptoms make interpretation difficult.
FDA also identifies potential injectable-peptide risks from aggregation, peptide-related impurities and immunogenicity.

Important notes

DSIP is not an FDA-approved medicine, and no matching DSIP or emideltide record appeared in the public ARTG search on 25 October 2026. Both listed suppliers describe their 10 mg product as research material, not for human consumption.
Prohibited in sport: DSIP falls within WADA S0 for pharmacological substances without current approval by a governmental regulatory health authority for human therapeutic use; WADA lists S0 as prohibited at all times.
FDA concluded that subcutaneous emideltide safety and effectiveness data are insufficient and highlighted possible immunogenicity and impurity-related risks.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

DSIP questions

How many units is 100 mcg of DSIP?

With the default 3 mL mix in a nominal 10 mg vial, the concentration is 3.33 mg/mL. A 100 mcg amount is 3 units on a U-100 syringe, or 0.03 mL.

How many units is 250 mcg of DSIP?

With the default 10 mg plus 3 mL mix, 250 mcg is 7.5 units on a U-100 syringe, which is 0.075 mL.

How much bacteriostatic water is used for DSIP 10 mg?

The default mix is 3 mL because Peak's DSIP page and the attributed reference protocol use that volume. It produces 3.33 mg/mL, where 30 U-100 units equals 1 mg or 0.30 mL.

How long does a 10 mg DSIP vial last?

A nominal 10 mg vial contains 100 amounts of 100 mcg, 50 amounts of 200 mcg, or about 33 amounts of 300 mcg. The number of days depends on the attributed step-up schedule and any material lost during preparation.

07 · SOURCES