Dosage guide · 2 mg vial

PEG-MGF Dosage Protocol

PEGylated mechano growth factor · IGF-1 splice-variant analogue

The reference protocol starts at 200 mcg once daily for weeks 1–2, then increases by 100 mcg every 2 weeks to 500 mcg in weeks 7–8. With 3 mL of bacteriostatic water, a 2 mg vial is 0.667 mg/mL, so 200 mcg is 30 units (0.30 mL) on a U-100 syringe. It is a research-only protocol, not an established human dose.

How oftenOnce daily

Starting dose200 mcg

Start = units30 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

PEG-MGF 2 mg vial

Not currently stocked by Peak Peptides Australia

No product photo — this strength isn't stocked by Peak

Dosage chart How to mix Supplies Calculator About PEG-MGF References

01 · DOSAGE CHART

PEG-MGF dosage chart

Gradual daily approach · 2 mg vial + 3 mL BAC water

BAC water added

Concentration0.67 mg/mL

1 unit (U-100)6.7 mcg= 0.01 mL

How oftenOnce dailySubcutaneous injection

3 mL is the mix used by the attributed 2 mg reference protocol, giving 0.667 mg/mL and 30 units = 200 mcg.

PEG-MGF dosage chart for a 2 mg vial mixed with 3 mL of bacteriostatic water
Week	Daily dose	Syringe units
Weeks 1–2Start	200 mcg	30 units0.3 mL
Weeks 3–4	300 mcg	45 units0.45 mL
Weeks 5–6	400 mcg	60 units0.6 mL
Weeks 7–8Maintenance	500 mcg	75 units0.75 mL

Once daily · any consistent time · subcutaneous injection

The attributed protocol increases by 100 mcg every 2 weeks.

It presents an 8-week schedule; the chart amounts are not a clinically established human dose.

Community-reported reference-site amounts — PEG-MGF has no clinically established human dose and is not approved in Australia. Education only, not medical advice. Dose amounts adapted from PEG MGF 2 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 2 mg vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 0.67 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 0.67 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 2 mg vial mixed with 3 mL.

Length of protocol

10PEG-MGF 2 mg vials19.6 mg needed in total

661 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

3BAC water 10 mL bottles30 mL used for mixing

132Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

0.67 mg/mL

(667 mcg/mL)

Calculated Liquid Volume

0.300 mL

Equivalent U-100 volume scale position

30markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About PEG-MGF

Protocol overview

Starting dose: 200 mcg once daily for weeks 1–2
How it increases: Add 100 mcg every 2 weeks to 500 mcg in weeks 7–8
Frequency: Once daily
When to take it: Any consistent time of day
Cycle length: 8 weeks in the attributed protocol
Common mix: 3 mL bacteriostatic water → 0.667 mg/mL

Storage

Before mixing: The attributed protocol says to keep the lyophilised vial frozen at −20 °C, dry and away from light.
After mixing: The attributed protocol says to refrigerate at 2–8 °C and protect it from light.
Never: Avoid repeated freeze–thaw cycles.

How it works

PEG-MGF is marketed as a PEGylated version of mechano growth factor, an IGF-1 gene splice variant also called IGF-1Ec. PEGylation is intended to alter how long a molecule remains in the body, but this does not establish the behaviour or benefit of a PEG-MGF product in people.

In human muscle biopsies after exercise-induced damage, MGF mRNA rose rapidly and transiently. Cell and animal experiments suggest synthetic MGF E-peptide analogues can affect muscle progenitor cells, but their receptor, processing and wider effects remain uncertain.

Illustrated mechanism explainer

What the research shows

In a study of 10 healthy men with exercise-induced muscle damage, MGF mRNA rose rapidly and transiently; the signalling tests in that report were done in C2C12 muscle-like cells, not with PEG-MGF dosing in people.
A primary-cell experiment found that the 24-amino-acid MGF E peptide increased proliferative lifespan in neonatal and young-adult human muscle-cell cultures, but not in cells from older adults. This was not a PEG-MGF clinical study.
In a 2019 mouse injury model, MGF injection did not protect muscle-fibre regeneration; it reduced fibrosis in the macrophage-depleted injury model. Molecular analyses used groups of 8 mice and regeneration analyses used groups of 4 mice.
The TGA ARTG public search for PEG-MGF returned no matching results. PEG-MGF is not an approved medicine in Australia, and no FDA-approved PEG-MGF label was identified.
A review concluded that endogenous short MGF E peptides had not been confirmed and that the mechanism and wider biological effects of these synthetic peptide mimetics remained incompletely understood.

Reported side effects

Human safety data for injected PEG-MGF at the chart amounts have not been established, so the frequency and severity of adverse effects are unknown.
In 2 black-market MGF preparations analysed by mass spectrometry, the products were C-terminally amidated analogues rather than the reference human MGF sequence, illustrating an identity and quality risk in unregulated products.
Growth-factor signalling and the broader biological effects of MGF E-peptide mimetics remain uncertain; cell and animal findings cannot establish safety in people.

Important notes

No PEG-MGF medicine was found in the TGA ARTG public search. It is not approved for human therapeutic use in Australia.
Prohibited in sport: WADA lists IGF-1 and its analogues, plus other growth factors or modulators affecting muscle or regenerative processes, under S2 at all times. Athletes should obtain advice from their anti-doping organisation.
There is no clinically established human dose for PEG-MGF. The chart copies the attributed educational reference protocol rather than a medicine label or human dose-escalation study.
Unregulated MGF products may not contain the claimed molecular form; a published analysis found amidated MGF analogues in 2 black-market preparations.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

PEG-MGF questions

How many units is 200 mcg of PEG-MGF in a 2 mg vial?

With 3 mL in a 2 mg vial, the concentration is 0.667 mg/mL. A 200 mcg amount is 30 units, or 0.30 mL, on a U-100 syringe.

How much bacteriostatic water is used for PEG-MGF 2 mg?

The attributed 2 mg protocol uses 3 mL, giving 0.667 mg/mL. At that concentration, 300 mcg is 45 units (0.45 mL), 400 mcg is 60 units (0.60 mL), and 500 mcg is 75 units (0.75 mL).

How long does a 2 mg PEG-MGF vial last?

At 200 mcg once daily, a 2 mg vial provides 10 doses. At 300 mcg it provides about 6.7 doses, at 400 mcg 5 doses, and at 500 mcg 4 doses.

Is PEG-MGF banned in sport?

WADA prohibits IGF-1 and its analogues, and also other growth factors or growth-factor modulators affecting muscle or regenerative processes, under S2 at all times.

07 · SOURCES