Dosage guide · 5 mg vial

MGF Dosage Protocol

Mechano growth factor · IGF-1 splice-variant research peptide

The reference protocol starts at 100 mcg once daily in week 1, then rises weekly to 150, 200, 250 and 300 mcg for weeks 5–8. With 3 mL of bacteriostatic water, a 5 mg vial is 1.67 mg/mL, so 100 mcg measures 6 units (0.06 mL) on a U-100 syringe.

How oftenOnce daily

Starting dose100 mcg

Start = units6 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

MGF 5 mg vial

Not currently stocked by Peak Peptides Australia

No product photo — this strength isn't stocked by Peak

Dosage chart How to mix Supplies Calculator About MGF References

01 · DOSAGE CHART

MGF dosage chart

Gradual daily approach · 5 mg vial + 3 mL BAC water

BAC water added

Concentration1.67 mg/mL

1 unit (U-100)16.7 mcg= 0.01 mL

How oftenOnce dailySubcutaneous injection

3 mL is the mix used in the cited 5 mg reference protocol, giving 1.67 mg/mL and 6 units for 100 mcg.

MGF dosage chart for a 5 mg vial mixed with 3 mL of bacteriostatic water
Week	Daily dose	Syringe units
Week 1Start	100 mcg	6 units0.06 mL
Week 2	150 mcg	9 units0.09 mL
Week 3	200 mcg	12 units0.12 mL
Week 4	250 mcg	15 units0.15 mL
Weeks 5–8Maintenance	300 mcg	18 units0.18 mL

Once daily · any consistent time · subcutaneous injection

The reference protocol increases by 50 mcg each week through week 5.

Its displayed chart ends at week 8; its accompanying text describes 8–12 weeks, with an optional extension to 16 weeks, without a separate later dose.

Reference-site educational amounts — MGF has no approved or clinically established human dose. Education only, not medical advice. Dose amounts adapted from MGF 5 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 5 mg vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 1.67 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 1.67 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 5 mg vial mixed with 3 mL.

Length of protocol

3MGF 5 mg vials13.3 mg needed in total

591 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle9 mL used for mixing

118Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

1.67 mg/mL

(1667 mcg/mL)

Calculated Liquid Volume

0.060 mL

Equivalent U-100 volume scale position

6markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About MGF

Protocol overview

Starting dose: 100 mcg once daily in week 1
How it increases: Add 50 mcg each week to 300 mcg in weeks 5–8
Frequency: Once daily
Route in source: Subcutaneous
Cycle length: 8 weeks on the displayed chart; source text describes 8–12 weeks, optionally 16
Common mix: 3 mL bacteriostatic water → 1.67 mg/mL

Storage

Before mixing: The cited protocol says −20 °C or colder for long-term storage, or about 4 °C for short-term storage.
After mixing: Refrigerate at 2–8 °C, protect from light and follow the preparation's validated use-by and sterile-handling instructions.
Never: Avoid repeated freeze–thaw cycles.

How it works

MGF is a term used for an IGF-1 splice variant, often called IGF-1Ec in humans. A small human exercise experiment found MGF messenger RNA rose 2.5 hours after resistance exercise in younger participants, but not older participants.

A synthetic 24-amino-acid MGF E-peptide has been studied in cells and animals. A review notes that an equivalent endogenous peptide has not been identified in cultured cells, animal tissues or biological fluids, so natural IGF-1 gene expression does not establish that injected synthetic peptide acts the same way.

Laboratory findings are inconsistent: one primary human-cell experiment reported increased satellite-cell activation and fusion, whereas another experiment found no proliferative effect at concentrations up to 500 ng/mL.

Illustrated mechanism explainer

What the research shows

In a human expression experiment, 8 young men and 7 older men completed 10 sets of 6 knee extensions at 80% of one-repetition maximum. Quadriceps samples 2.5 hours later showed a significant MGF mRNA rise in the younger group but not the older group; this did not administer MGF peptide.
In a rabbit bone-defect experiment, 3 groups of 9 received 0, 28.5 or 57 mcg/kg MGF-Ct24E for 5 days. At 8 weeks, 4 of 9 high-dose defects had radiographic healing versus 0 of 9 in either control or low-dose groups; animal results do not establish a human dose.
Primary human muscle-cell cultures exposed to MGF-E reported increased proliferation and fusion in neonatal and young-adult cells, but another in-vitro experiment found no increase in human myoblast proliferation with MGF peptide concentrations up to 500 ng/mL.
The TGA ARTG search for ‘Mechano Growth Factor’ returned no matching results. MGF is not an approved medicine in Australia, and no FDA-approved MGF medicine label is cited here.

Reported side effects

Published evidence reviewed here does not provide a human adverse-event rate or long-term safety profile for administered MGF, so risks cannot be quantified.
FDA says the related PEG-MGF formulation has no human exposure data and may pose immunogenicity risks from aggregation, peptide-related impurities and active-ingredient characterisation. This is a warning about PEG-MGF, not a measured risk rate for non-PEG MGF.

Important notes

MGF is not an approved medicine in Australia: the ARTG search for ‘Mechano Growth Factor’ returned no matching results. No FDA-approved MGF medicine label is cited here.
Prohibited in sport: WADA identifies MGFs as explicitly mentioned on its Prohibited List, and its current S2 growth-factor category includes IGF-1 and its analogues.
The amounts above are copied from an educational reference protocol, not from a clinically established MGF dose. Synthetic MGF findings are inconsistent and do not demonstrate muscle repair or performance benefit in people.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

MGF questions

How many units is 100 mcg of MGF?

With 3 mL in a 5 mg vial, the concentration is 1.67 mg/mL. A 100 mcg amount is 6 units on a U-100 syringe, which is 0.06 mL.

How much bacteriostatic water for a 5 mg MGF vial?

The cited 5 mg reference protocol uses 3 mL. That calculation gives 1.67 mg/mL, or about 16.7 mcg per U-100 syringe unit.

How many units is 300 mcg of MGF?

With the default 5 mg plus 3 mL mix, 300 mcg is 18 units on a U-100 syringe, equal to 0.18 mL.

How long does a 5 mg MGF vial last?

Ignoring preparation losses, a 5 mg vial contains 50 daily 100 mcg amounts, 25 daily 200 mcg amounts or about 16.7 daily 300 mcg amounts. The 3 mL default mix changes the volume drawn, not the total peptide amount.

07 · SOURCES