Dosage guide · 5000 IU vial

HCG Dosage Protocol

Human chorionic gonadotropin · glycoprotein hormone with LH-like activity

The reference protocol lists 500 IU three times weekly for weeks 1–12. With 2 mL of bacteriostatic water, a 5000 IU vial is 2500 IU/mL, so 500 IU is 20 units (0.20 mL) on a U-100 syringe.

How often3 times weekly

Starting dose500 IU

Start = units20 units

Mix2 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

HCG 5000 IU vial

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Dosage chart How to mix Supplies About HCG References

01 · DOSAGE CHART

HCG dosage chart

Standard intermittent protocol · 5000 IU vial + 2 mL BAC water

BAC water added

Concentration2500 IU/mL

1 unit (U-100)25 IU= 0.01 mL

How often3 times weeklySubcutaneous injection

2 mL is shown on both Peak's HCG page and the reference protocol, giving 2500 IU/mL.

HCG dosage chart for a 5000 IU vial mixed with 2 mL of bacteriostatic water
Week	Dose each time	Syringe units	Syringe fill
Weeks 1–12Start	500 IU	20 units0.2 mL

3 times weekly · consistent days · subcutaneous injection

The source chart uses three evenly spaced injection days each week.

Its example schedule is Monday, Wednesday and Friday.

Reference-site educational amounts — hCG is a prescription hormone medicine and the Peak research listing is not a TGA-approved therapeutic good. Education only, not medical advice. Dose amounts adapted from HCG 5000 IU vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 5000 IU vial with 2 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 2 full draws of bacteriostatic water gives 2500 IU/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 2 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 2500 IU/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 5000 IU vial mixed with 2 mL.

Length of protocol

3HCG 5000 IU vials12000 IU needed in total

271 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle6 mL used for mixing

54Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

HCG 5000 IU vialPeak Peptides Australia

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · PROTOCOL DETAILS

About HCG

Protocol overview

Starting dose: 500 IU per injection
Protocol: 500 IU three times weekly for weeks 1–12
Frequency: 3 times weekly
When to take it: Consistent, evenly spaced days
Common mix: 2 mL bacteriostatic water → 2500 IU/mL
Measurement: 500 IU = 20 U-100 units = 0.20 mL

Storage

Before mixing: Peak says unopened powder should be kept at 2–8 °C in the dark, or at −20 °C for longer-term storage.
After mixing: Peak says to keep a BAC-water mix upright at 2–8 °C, away from light, and use it within about 28 days.
Never: Do not freeze a mixed vial, or use it if it is cloudy or contains particles.

How it works

HCG, also written hCG, is a two-subunit glycoprotein hormone rather than a short synthetic peptide. It and luteinising hormone use the same LH/CG receptor.

The prescription Pregnyl label says hCG acts much like pituitary LH: it stimulates Leydig cells in the testes to produce androgens and the ovarian corpus luteum to produce progesterone. These hormonal effects require medical oversight.

Illustrated mechanism explainer

What the research shows

In a randomised 3-week trial of 29 healthy men receiving 200 mg testosterone enanthate weekly, intratesticular testosterone fell 94% with placebo; it was 7% below baseline with 250 IU hCG every other day and 26% above baseline with 500 IU every other day.
The US Pregnyl label gives medical regimens for selected male hypogonadotropic hypogonadism of 500–1000 USP units three times weekly for 3 weeks, then the same amount twice weekly for 3 weeks. Those are product-label regimens, not a protocol for Peak's research listing.
The TGA records CHORIOMON 5000 I.E. as a current Section 19A approved medicine until 31 August 2027 in the shortage context for Pregnyl 5000 IU; this approval is product-specific.
A 1995 meta-analysis located 8 controlled and 16 uncontrolled hCG-obesity trials and concluded that hCG did not cause weight loss, fat redistribution, reduced hunger or improved wellbeing.

Reported side effects

Prescription hCG labels report injection-site bruising, pain, redness, swelling or itching, as well as headache, fatigue and oedema.
Hypersensitivity reactions, including anaphylaxis, have been reported with urinary-derived hCG products.
In males, the Pregnyl label says water and sodium retention can occur after high doses and gynaecomastia may occur sporadically.
When hCG is used with gonadotropins for ovarian stimulation, ovarian hyperstimulation syndrome can be life-threatening and thromboembolic events have been reported.

Important notes

Human chorionic gonadotropin is a prescription medicine in Australia in products such as Pregnyl and Ovidrel. Peak's 5000 IU listing says it is for in-vitro research only and is not a TGA-approved therapeutic good.
Prohibited in sport for males: WADA lists chorionic gonadotrophin under S2.2.1 testosterone-stimulating peptides.
HCG has hormonal effects and prescription use needs appropriate clinical assessment and monitoring; it is not a weight-loss treatment.
Peak's page reports a product-specific Janoshik result of 4556 IU for Task 135353, rather than the 5000 IU label. Calculator values use the labelled strength, so this discrepancy matters.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

05 · QUICK ANSWERS

HCG questions

How many U-100 units is 500 IU of HCG?

With 2 mL in a 5000 IU vial, the concentration is 2500 IU/mL. A 500 IU amount is 20 U-100 units, which is 0.20 mL.

How much BAC water for HCG 5000 IU?

The default mix is 2 mL of bacteriostatic water. It gives 2500 IU/mL, where 1 U-100 unit is 25 IU.

How long does a 5000 IU HCG vial last?

At the chart amount of 500 IU, a 5000 IU vial contains 10 injections. At 3 injections a week, that is about 3.3 weeks of supply.

Is HCG a prescription medicine in Australia?

Yes. Prescription hCG medicine contexts include Pregnyl and Ovidrel. The Peak listing is a separate in-vitro research product and says it is not a TGA-approved therapeutic good.

06 · SOURCES