Dosage guide · 10 mg vial

AOD-9604 Dosage Protocol

hGH fragment 176–191 · modified growth-hormone fragment

The reference protocol uses 300 mcg once daily for weeks 1–4, then 500 mcg once daily for weeks 5–12. Peak's 10 mg vial uses 2 mL of its supplied acidic solution by default, making 5 mg/mL; 300 mcg is 6 units on a U-100 syringe.

How oftenOnce daily

Starting dose300 mcg

Start = units6 units

Mix2 mL acidic diluent

Reviewed 25 Oct 2026 · Education only, not medical advice

AOD-9604 10 mg vial

View on Peak Peptides Also at Pure Performance

Exact Peak Peptides Australia product photo

Dosage chart How to mix Supplies Calculator About AOD-9604 References

01 · DOSAGE CHART

AOD-9604 dosage chart

Gradual daily approach · 10 mg vial + 2 mL acidic diluent

Acidic diluent added

Concentration5 mg/mL

1 unit (U-100)50 mcg= 0.01 mL

How oftenOnce dailySubcutaneous injection

Peak supplies product-specific acidic solution and highlights 2 mL, which makes the labelled concentration 5 mg/mL.

This product is mixed with the acidic diluent Peak supplies, not bacteriostatic water. Why acidic diluents are used

AOD-9604 dosage chart for a 10 mg vial mixed with 2 mL of the acidic diluent Peak supplies
Week	Daily dose	Syringe units	Syringe fill
Weeks 1–4Start	300 mcg	6 units0.06 mL
Weeks 5–12Maintenance	500 mcg	10 units0.1 mL

Once daily · morning noted by source · subcutaneous injection

The reference-site schedule increases from 300 mcg to 500 mcg from week 5.

The cited protocol uses a 12-week course and notes morning timing; a preferred timing is not established by human evidence.

Community-reported reference amounts — AOD-9604 has no approved or clinically established human subcutaneous dose. Education only, not medical advice. Dose amounts adapted from AOD-9604 10 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 10 mg vial with 2 mL of acidic diluent

Using a 1 mL (100-unit) insulin syringe, 2 full draws of the acidic diluent Peak supplies gives 5 mg/mL.

Use the acidic diluent Peak supplies for this product, not bacteriostatic water — the steps below are the same apart from the liquid. Read the acidic-diluent guide.

1
Add the diluent slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 2 mL of the acidic diluent Peak supplies and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 5 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 10 mg vial mixed with 2 mL.

Length of protocol

1AOD-9604 10 mg vial8.4 mg needed in total

291 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1Acidic diluent (10 mL)2 mL used for mixing

58Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies. Peak supplies the acidic diluent for this product.

AOD-9604 10 mg vialPeak Peptides Australia AOD-9604 10 mg vialPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume2 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

5.00 mg/mL

(5000 mcg/mL)

Calculated Liquid Volume

0.060 mL

Equivalent U-100 volume scale position

6markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About AOD-9604

Protocol overview

Starting dose: 300 mcg once daily for weeks 1–4
How it increases: 500 mcg once daily for weeks 5–12
Frequency: Once daily
When to take it: Morning is noted by the reference page; human evidence does not establish preferred timing
Course length: 12 weeks in the reference protocol
Default mix: 2 mL supplied acidic solution → 5 mg/mL

Storage

Before mixing: Keep the unopened powder cold and protected from light; Peak states 2–8 °C, with −20 °C for long-term storage.
After mixing: Refrigerate at 2–8 °C, upright and away from light; follow the supplied acidic solution's label for the use period.
Never: Do not substitute another solvent or freeze a mixed vial.

How it works

AOD-9604 is a modified 16-amino-acid fragment based on the C-terminal region of human growth hormone, not full-length growth hormone.

In obese mice, chronic 14-day treatment was associated with reduced body-weight gain and body fat, increased fat oxidation and changes in beta-3 adrenergic receptor RNA. Those are animal findings and do not establish effects in people or for a subcutaneous research vial.

Illustrated mechanism explainer

What the research shows

A 2001 mouse study reported reduced body weight and body fat after 14 days of chronic AOD-9604 treatment; effects did not occur in beta-3 adrenergic receptor knockout mice in the same chronic experiment.
A 2013 report summarised six randomised, double-blind, placebo-controlled studies involving 893 adults; the study products were intravenous or oral, not subcutaneous.
FDA's 2024 review found no human subcutaneous exposure information. It described a 23-person intravenous study using 25, 50 or 100 mcg/kg once weekly for 4 weeks, where weight loss was not statistically different from placebo.
FDA's review described a 300-person oral study of 1, 5, 10, 20 or 30 mg daily for 12 weeks and concluded the cited clinical studies did not show statistically significant weight loss versus placebo. AOD-9604 is not an approved medicine in Australia or the United States.

Reported side effects

There are no human subcutaneous safety data for AOD-9604, according to FDA's 2024 review.
FDA reported serious adverse events in oral studies including diarrhoea, chest tightness and various cancers; it did not establish that the events were caused by AOD-9604.
In an intravenous study summarised in the 2013 report, the most common reported adverse event was headache, occurring 6 times; none was severe and the profile was similar across AOD-9604 and placebo treatments.
FDA noted possible peptide immunogenicity and nonclinical signals concerning bone health in rats after 13 weeks and possible liver toxicity in monkeys after 9 months.

Important notes

AOD-9604 is not an approved medicine in Australia or the United States; Peak and Pure list these 10 mg products for research only, not human consumption.
Prohibited in sport at all times: the 2026 WADA List names AOD-9604 as a growth-hormone fragment under S2.2.3.
The TGA warns that unapproved peptide products may be poorly labelled and not subject to Australian safety or quality standards.
Peak's 10 mg format requires its supplied acidic solution; do not transfer a bacteriostatic-water instruction from another product or supplier.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

AOD-9604 questions

How many units is 300 mcg of AOD-9604?

With the 10 mg vial and 2 mL default mix, the concentration is 5 mg/mL. 300 mcg is 6 units, or 0.06 mL, on a U-100 syringe.

How many units is 500 mcg of AOD-9604?

With the 10 mg vial and 2 mL default mix, 500 mcg is 10 units, or 0.10 mL, on a U-100 syringe.

How much solution is used for Peak's AOD-9604 10 mg vial?

Peak states that this format must use its supplied acidic solution. The default 2 mL mix gives 5 mg/mL, so 1 mg is 20 units or 0.20 mL on a U-100 syringe.

How long does a 10 mg AOD-9604 vial last?

By label arithmetic, a 10 mg vial contains about 33.3 daily 300 mcg amounts or 20 daily 500 mcg amounts. The full 12-week reference schedule totals 36.4 mg, before allowing for any preparation loss.

07 · SOURCES