Dosage guide · 10 mg blend

Wolverine Stack Dosage Protocol

BPC-157 + TB-500 fixed-ratio research blend

The reference schedule is once daily: 500 mcg total blend for weeks 1–2, 666 mcg for weeks 3–4, then 1,000 mcg for weeks 5–8. With 3 mL bacteriostatic water, the 10 mg vial is 3.33 mg/mL, so the 500 mcg starting amount is 15 units (0.15 mL) on a U-100 syringe. Each 500 mcg total amount contains 250 mcg of each component.

How oftenOnce daily

Starting dose250 mcg each

Start = units15 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

Wolverine Stack 10 mg blend vial

View on Peak Peptides Also at Pure Performance

Exact Peak Peptides Australia product photo

Dosage chart How to mix Supplies Calculator About Wolverine Stack References

01 · DOSAGE CHART

Wolverine Stack dosage chart

Gradual daily blend approach · 10 mg blend + 3 mL BAC water

BAC water added

Concentration3.33 mg/mL total1.67 mg/mL of each peptide

1 unit (U-100)16.7 mcg of each= 0.01 mL

How oftenOnce dailySubcutaneous injection

3 mL is the mix shown on Peak's page and the attributed 10 mg blend reference, giving 3.33 mg/mL total blend.

Wolverine Stack dosage chart for a 10 mg blend vial mixed with 3 mL of bacteriostatic water
Week	Dose of each peptide	Syringe units
Weeks 1–2Start	250 mcg500 mcg total	15 units0.15 mL
Weeks 3–4	333 mcg666 mcg total	20 units0.2 mL
Weeks 5–8Maintenance	500 mcg1 mg total	30 units0.3 mL

Once daily · any consistent time · subcutaneous injection

Amounts are total blend amounts: each draw contains equal masses of BPC-157 and TB-500.

The attributed standard calculation sequence runs for 8 weeks.

Community-reported amounts — this BPC-157 plus TB-500 blend has no approved or clinically established human dose. Education only, not medical advice. Dose amounts adapted from BPC-157 plus TB-500 10 mg blend dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 10 mg blend vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 3.33 mg/mL in total.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 3.33 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 10 mg blend vial mixed with 3 mL.

Length of protocol

2Wolverine Stack 10 mg blend vials16.32 mg needed in total

301 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle6 mL used for mixing

60Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Wolverine Stack 10 mg blend vialPeak Peptides Australia Wolverine Stack 10 mg blend vialPure Performance Australia

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter the combined total of all 2 peptides (for example 200 mcg = 100 mcg of each).

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

3.33 mg/mL

(3333 mcg/mL)

Calculated Liquid Volume

0.150 mL

Equivalent U-100 volume scale position

15markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About Wolverine Stack

Protocol overview

Starting dose: 500 mcg total blend once daily, or 250 mcg of each component
How it increases: 666 mcg total in weeks 3–4, then 1,000 mcg total in weeks 5–8
Frequency: Once daily
When to take it: Any consistent time of day; no specific timing is stated in the source
Cycle length: The attributed standard calculation sequence runs for 8 weeks
Common mix: 3 mL bacteriostatic water → 3.33 mg/mL total blend

Storage

Before mixing: Keep the sealed vial protected from moisture, light and repeated temperature changes; follow the product label.
After mixing: Refrigerate at 2–8 °C away from light; Peak says to use within about 4 weeks (28 days).
Never: Never freeze the prepared solution or use it if it is cloudy or has particles.

How it works

Wolverine Stack is a fixed 1:1 blend of BPC-157 and TB-500, rather than a new single molecule. BPC-157 is a 15-amino-acid peptide whose tissue-repair findings are mainly from laboratory and animal research.

TB-500 is not full-length thymosin beta-4: analytical work identified it as the N-terminal acetylated 17–23 fragment, Ac-LKKTETQ. Human work with recombinant full-length thymosin beta-4 does not establish effects for TB-500 or for this premixed blend.

Illustrated mechanism explainer

What the research shows

A 2025 systematic review of BPC-157 included 36 studies: 35 preclinical studies and 1 clinical study. The clinical report was a retrospective series in which 7 of 12 people reported more than 6 months of relief after an intra-articular injection; the review found no clinical safety data.
A first-in-human trial of recombinant full-length thymosin beta-4, not TB-500, gave single intravenous doses of 0.05–25 micrograms/kg to 54 healthy people. A separate 30-person multiple-dose group received 0.5, 2.0 or 5.0 micrograms/kg daily for 10 days.
The 2026 WADA List places BPC-157 in S0 and names thymosin beta-4 derivatives including TB-500 in S2; both categories are prohibited at all times.
The TGA identifies products containing BPC-157 and TB-500 as examples of unapproved peptides that have not been evaluated for safety, quality or effectiveness.

Reported side effects

Human safety information for this premixed BPC-157 plus TB-500 product is not established; the BPC-157 review found no clinical safety data.
The TGA reports serious adverse effects associated with unapproved peptide products, including liver damage, severe allergic reactions requiring hospitalisation, and inflammation or other complications requiring medical attention.
In the full-length thymosin beta-4 intravenous trial, adverse events were mild to moderate, with no dose-limiting toxicities or serious adverse events; this does not establish safety for TB-500 or this blend.

Important notes

Wolverine Stack is not an approved medicine in Australia; the TGA lists BPC-157 and TB-500 among unapproved peptide products.
Prohibited in sport: WADA lists BPC-157 under S0 and thymosin beta-4 derivatives including TB-500 under S2, prohibited at all times.
The 10 mg label is total blend content, not 10 mg of each component: it is 5 mg BPC-157 plus 5 mg TB-500 at a fixed ratio.
The TGA warns that unapproved peptides may have unknown composition, quality, sterility and side-effect risks, including counterfeit or poorly labelled products.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

Wolverine Stack questions

How many units is 500 mcg of Wolverine Stack?

With 3 mL in a 10 mg blend vial, the total concentration is 3.33 mg/mL. A 500 mcg total-blend amount is 15 units, or 0.15 mL, on a U-100 syringe and contains 250 mcg BPC-157 plus 250 mcg TB-500.

Does Wolverine Stack 10 mg mean 10 mg of each peptide?

No. The 10 mg label is the combined amount: 5 mg BPC-157 plus 5 mg TB-500. Because the blend is fixed at an equal mass ratio, a 1,000 mcg total-blend amount contains 500 mcg of each component.

How much BAC water for Wolverine Stack 10 mg?

This page defaults to 3 mL because Peak and the attributed 10 mg reference show that volume. It makes 3.33 mg/mL total blend, so 30 U-100 units is 1 mg total blend. Pure's supplied product page instead shows 2 mL, which would make 5 mg/mL and change all syringe-unit figures.

How long does a 10 mg Wolverine Stack vial last?

A 10 mg blend vial contains 20 amounts of 500 mcg total blend, about 15 amounts of 666 mcg, or 10 amounts of 1,000 mcg. At once daily, that is 20 days, about 15 days, or 10 days respectively; this is vial-yield arithmetic, not a duration recommendation.

07 · SOURCES