Dosage guide · 50 mg vial

GHK-Cu Dosage Protocol

Copper tripeptide-1 · glycyl-histidyl-lysine copper complex

The GHK-Cu reference protocol starts at 1 mg once daily, five days a week for weeks 1–4, then steps to 1.5 mg and 2 mg. With 3 mL in a 50 mg vial, the concentration is 16.67 mg/mL, so the 1 mg starting amount is 6 U-100 units (0.06 mL).

How oftenOnce daily, 5 days

Starting dose1 mg

Start = units6 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

GHK-Cu 50 mg vial

View on Peak Peptides

Exact Peak Peptides Australia product photo

Dosage chart How to mix Supplies Calculator About GHK-Cu References

01 · DOSAGE CHART

GHK-Cu dosage chart

Gradual daily approach · 50 mg vial + 3 mL BAC water

BAC water added

Concentration16.67 mg/mL

1 unit (U-100)166.7 mcg= 0.01 mL

How oftenOnce daily, 5 daysSubcutaneous injection

3 mL is the reference protocol and Peak guide mix, giving 16.67 mg/mL and 6 units for 1 mg.

GHK-Cu dosage chart for a 50 mg vial mixed with 3 mL of bacteriostatic water
Week	Dose each time	Syringe units
Weeks 1–4Start	1 mg	6 units0.06 mL
Weeks 5–8	1.5 mg	9 units0.09 mL
Weeks 9–12+Maintenance	2 mg	12 units0.12 mL

Once daily, 5 days each week · subcutaneous injection

The reference schedule steps up after each four-week block.

The reference page shows the 2 mg amount from week 9 onward and gives supply examples for 8, 12 and 16 weeks.

Peak's listing states its vial is for in-vitro research and not intended for injection; see Important notes.

Reference-site, community-reported injection amounts — GHK-Cu has no approved or clinically established injectable human dose. Education only, not medical advice. Dose amounts adapted from GHK-Cu 50 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 50 mg vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 16.67 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 16.67 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 50 mg vial mixed with 3 mL.

Length of protocol

1GHK-Cu 50 mg vial50 mg needed in total

411 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle3 mL used for mixing

82Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

GHK-Cu 50 mg vialPeak Peptides Australia

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

Concentration

16.67 mg/mL

(16667 mcg/mL)

Calculated Liquid Volume

0.060 mL

Equivalent U-100 volume scale position

6markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About GHK-Cu

Protocol overview

Starting dose: 1 mg once daily, 5 days each week
How it increases: 1.5 mg in weeks 5–8, then 2 mg from week 9
Frequency: Once daily, 5 days each week
When to take it: Any consistent time on the five scheduled days
Reference length: 12 weeks then ongoing; the reference gives 8, 12 and 16-week supply examples
Common mix: 3 mL BAC water → 16.67 mg/mL

Storage

Before mixing: Keep unopened powder in the fridge at 2–8 °C and in the dark; Peak's guide says −20 °C may be used for long-term storage.
After mixing: Keep upright at 2–8 °C and protected from light; Peak's guide says to use within about 28 days after mixing with BAC water.
Never: Do not freeze the mixed vial or use a solution that is cloudy, discoloured or has unexpected particles.

How it works

GHK-Cu is a copper complex of the naturally occurring three-amino-acid sequence glycyl-histidyl-lysine, also called copper tripeptide-1.

Cell, tissue and animal research has examined its effects on extracellular-matrix turnover, collagen-related signals, fibroblasts and keratinocytes. These findings do not establish effects or a dose for an injectable GHK-Cu vial.

Published human findings cited in reviews concern topical creams, not this reconstituted 50 mg research vial.

Illustrated mechanism explainer

What the research shows

A 2015 review described a one-month topical thigh-cream comparison in which collagen increases were reported in 70% of women using GHK-Cu cream, versus 50% using vitamin C cream and 40% using retinoic acid cream.
The same review described a 12-week facial-cream study in 71 women, a 12-week eye-cream comparison in 41 women, and a 12-week twice-daily facial-cream study in 67 women. These are topical-formulation findings, not injection data.
The 2018 review describes GHK-Cu as a naturally occurring copper-binding tripeptide and summarises mainly cell, animal and topical skin research.
The TGA names GHK-Cu as an example of an unapproved peptide product not included in the ARTG; these products have not been evaluated by the TGA for safety, quality or effectiveness.
FDA says compounded injectable GHK-Cu may pose immunogenicity risk from aggregation and peptide-related impurities, and that human data informing safety considerations are limited.

Reported side effects

There is no established adverse-effect rate for injectable GHK-Cu; FDA says human data informing injectable safety considerations are limited.
FDA identifies potential immunogenicity risk from aggregation and peptide-related impurities in compounded injectable GHK-Cu.
The TGA warns that unapproved injected peptide products can involve contamination, infection or local tissue damage, and may have unknown contents, sterility and side effects. These are product and route risks, not quantified GHK-Cu-specific event rates.
Topical cream findings cannot determine the side-effect profile of a reconstituted injectable vial.

Important notes

GHK-Cu is not included in the Australian Register of Therapeutic Goods. The TGA has not evaluated unapproved peptide products for safety, quality or effectiveness.
Peak's 50 mg product page says the product is supplied strictly for in-vitro laboratory research and is not intended for human or animal consumption or injection. This conflicts with the reference site's injection schedule.
The 2026 WADA Prohibited List does not name GHK-Cu. Its S0 category prohibits pharmacological substances with no current approval by any governmental regulatory health authority; athletes should obtain sport-specific anti-doping advice before use.
Product labelling may be inaccurate or misleading, according to the TGA; a vial's stated strength does not establish sterility, identity or suitability for injection.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

GHK-Cu questions

How many units is 1 mg of GHK-Cu from a 50 mg vial?

With 3 mL in a 50 mg vial, the concentration is 16.67 mg/mL. A 1 mg amount is 6 U-100 units, which is 0.06 mL.

How many units are 1.5 mg and 2 mg of GHK-Cu?

With the 3 mL default mix in a 50 mg vial, 1.5 mg is 9 U-100 units or 0.09 mL. A 2 mg amount is 12 U-100 units or 0.12 mL.

How much BAC water is used for a 50 mg GHK-Cu vial?

The default reference mix is 3 mL, which gives 16.67 mg/mL. The selector also provides 2 mL and 4 mL options, changing concentration and units but not the 50 mg vial content.

How long does a 50 mg GHK-Cu vial last?

At five administrations a week, 50 mg supplies 10 weeks at 1 mg, about 6.7 weeks at 1.5 mg, or 5 weeks at 2 mg. The stepped reference schedule uses 50 mg over its first 8 weeks.

07 · SOURCES