Dosage guide · 50 mg vial

5-Amino-1MQ Dosage Protocol

5-amino-1-methylquinolinium · small-molecule NNMT inhibitor

The reference schedule starts at 2.5 mg once daily for days 1–2, then uses 5 mg once daily from day 3. With 4 mL of bacteriostatic water, a 50 mg vial is 12.5 mg/mL, so the 2.5 mg start is 20 units (0.20 mL) on a U-100 syringe. This is an unvalidated injectable research schedule, not an oral capsule guide.

How oftenOnce daily

Starting dose2.5 mg

Start = units20 units

Mix4 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

5-Amino-1MQ 50 mg vial

Not currently stocked by Peak Peptides Australia

No product photo — this strength isn't stocked by Peak

Dosage chart How to mix Supplies Calculator About 5-Amino-1MQ References

01 · DOSAGE CHART

5-Amino-1MQ dosage chart

Daily reference schedule · 50 mg vial + 4 mL BAC water

BAC water added

Concentration12.5 mg/mL

1 unit (U-100)125 mcg= 0.01 mL

How oftenOnce dailySubcutaneous injection

4 mL is the mix used by the 50 mg reference protocol, making 2.5 mg equal 20 units.

5-Amino-1MQ dosage chart for a 50 mg vial mixed with 4 mL of bacteriostatic water
Days	Daily dose	Syringe units	Syringe fill
Days 1–2Start	2.5 mg	20 units0.2 mL
Day 3 onwardsMaintenance	5 mg	40 units0.4 mL

Once daily · subcutaneous injection

The 5 mg once-daily amount begins on day 3; the day labels preserve the source timing.

The reference also lists 2.5 mg twice daily as an alternative split of the same 5 mg total daily amount.

Community-reported reference amounts — 5-Amino-1MQ has no approved or clinically established human dose. Education only, not medical advice. Dose amounts adapted from 5-Amino-1MQ 50 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 50 mg vial with 4 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 4 full draws of bacteriostatic water gives 12.5 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 4 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 12.5 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 50 mg vial mixed with 4 mL.

Length of protocol

25-Amino-1MQ 50 mg vials65 mg needed in total

161 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle8 mL used for mixing

32Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume4 mL

Amount to Evaluate (not a recommendation)

Concentration

12.50 mg/mL

(12500 mcg/mL)

Calculated Liquid Volume

0.200 mL

Equivalent U-100 volume scale position

20markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About 5-Amino-1MQ

Protocol overview

Starting dose: 2.5 mg once daily for days 1–2
How it increases: 5 mg once daily from day 3
Frequency: Once daily in the primary reference schedule
When to take it: No timing has been clinically established in people
Cycle length: No clinically established cycle length
Common mix: 4 mL bacteriostatic water → 12.5 mg/mL

Storage

Before mixing: The reference protocol specifies lyophilised material at −20 °C, protected from light.
After mixing: The reference protocol specifies 2–8 °C, protected from light; follow the formulation-specific use-by period.
Never: Do not use it if the solution is cloudy or has particles.

How it works

5-Amino-1MQ, also called 5-amino-1-methylquinolinium, is a small molecule rather than a peptide. It was developed to inhibit nicotinamide N-methyltransferase, or NNMT.

NNMT methylates nicotinamide. Cell and mouse experiments found that blocking NNMT changed metabolic co-factors and fat-tissue measures, but these findings do not establish a human effect, dose or safety profile.

Illustrated mechanism explainer

What the research shows

In an 11-day diet-induced-obesity mouse experiment, nine mice received 20 mg/kg by subcutaneous injection three times daily, about 34 mg/kg/day. The treated mice lost 2.0 ± 0.6 g, or about 5.1% of baseline, while controls gained 0.6 ± 0.4 g; this is animal evidence only.
A 30-day mouse experiment assigned eight animals each to vehicle, 10 mg/kg/day or 32 mg/kg/day subcutaneous treatment. The high-dose group gained 0.9 g versus 5.4 g in controls, without a significant treatment effect on food intake; it does not provide a human dose.
In rats, the pharmacokinetic paper reported 38.4% oral bioavailability and terminal half-lives of 6.90 ± 1.20 hours after oral dosing and 3.80 ± 1.10 hours after intravenous dosing. These are not human values.
The exact-term ClinicalTrials.gov search returned zero records for 5-Amino-1MQ, so no registered human clinical dose or safety result was identified.
The TGA ARTG exact-name search returned no matching result for 5-Amino-1MQ. It is not an approved medicine for human therapeutic use in Australia.

Reported side effects

No human clinical adverse-effect profile has been established; the exact-term ClinicalTrials.gov search returned zero records.
In the initial mouse dose-escalation work, up to 60 mg/kg/day was described as well tolerated with no observable adverse effects, but animal observations cannot demonstrate human safety.
The TGA warns that poorly labelled, unapproved injectable products can have unverifiable contents, concentration, sterility, contaminants or toxins.

Important notes

Not approved for human therapeutic use in Australia: the TGA ARTG exact-name search returned no matching result.
Prohibited in sport: WADA class S0 prohibits pharmacological substances without current approval by a governmental health authority for human therapeutic use.
There is no registered human clinical dose, safety profile, treatment duration or oral schedule for 5-Amino-1MQ.
The TGA warns that unapproved injectable products that are poorly labelled may have unverifiable contents, strength, sterility or contaminants.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS

5-Amino-1MQ questions

How many units is 2.5 mg of 5-Amino-1MQ?

With 4 mL in a 50 mg vial, the concentration is 12.5 mg/mL. A 2.5 mg amount is 20 units, or 0.20 mL, on a U-100 syringe. A 5 mg amount is 40 units, or 0.40 mL.

How much bacteriostatic water is used for a 50 mg vial?

The 50 mg reference protocol uses 4 mL of bacteriostatic water, giving 12.5 mg/mL. Peak does not stock this product and no Peak-specific mixing direction was supplied.

How long does a 50 mg vial last?

At 2.5 mg once daily, a 50 mg vial contains 20 daily amounts. At 5 mg once daily, it contains 10 daily amounts. Splitting 5 mg/day as 2.5 mg twice daily still uses 10 days of material.

Is 5-Amino-1MQ oral or injectable?

This 50 mg guide is injectable because the matching reference page specifies reconstitution with 4 mL bacteriostatic water and subcutaneous administration. Rat oral bioavailability data do not establish an oral human dose or schedule.

07 · SOURCES