Dosage guide · 10 mg vial

Sermorelin Dosage Protocol

GHRH(1-29) analogue · growth hormone-releasing factor

The reference-site chart uses 200 mcg nightly for weeks 1–2, then 300 mcg, 400 mcg and 500 mcg in two-week steps through week 8. With 3 mL of bacteriostatic water, a 10 mg vial is 3.33 mg/mL, so the 200 mcg starting amount is 6 units (0.06 mL) on a U-100 syringe.

How oftenOnce nightly

Starting dose200 mcg

Start = units6 units

Mix3 mL BAC water

Reviewed 25 Oct 2026 · Education only, not medical advice

Sermorelin 10 mg vial

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Dosage chart How to mix Supplies Calculator About Sermorelin References

01 · DOSAGE CHART

Sermorelin dosage chart

Gradual nightly approach · 10 mg vial + 3 mL BAC water

BAC water added

Concentration3.33 mg/mL

1 unit (U-100)33.3 mcg= 0.01 mL

How oftenOnce nightlySubcutaneous injection

3 mL is the reference mix shown by PeptideDosages and on Peak's product page, giving 3.33 mg/mL.

Sermorelin dosage chart for a 10 mg vial mixed with 3 mL of bacteriostatic water
Week	Daily dose	Syringe units
Weeks 1–2Start	200 mcg	6 units0.06 mL
Weeks 3–4	300 mcg	9 units0.09 mL
Weeks 5–6	400 mcg	12 units0.12 mL
Weeks 7–8Maintenance	500 mcg	15 units0.15 mL

Once nightly · bedtime · subcutaneous injection

The attributed reference chart increases by 100 mcg every 2 weeks and ends at week 8.

The reference chart specifies bedtime administration once daily.

Reference-site calculation amounts — Sermorelin has no current TGA- or FDA-approved adult dosing schedule. Education only, not medical advice. Dose amounts adapted from Sermorelin 10 mg vial dosage protocol (PeptideDosages.com); units are recalculated for the vial and mix selected above.

02 · HOW TO MIX

Mixing a 10 mg vial with 3 mL of BAC water

Using a 1 mL (100-unit) insulin syringe, 3 full draws of bacteriostatic water gives 3.33 mg/mL.

1
Add the water slowly
Wipe both rubber stoppers with an alcohol swab and let them dry. Draw 3 mL of bacteriostatic water and let it run slowly down the inside of the vial wall — not straight onto the powder.
2
Swirl gently
Swirl or roll the vial gently until the powder has dissolved. Give it a few minutes to settle if needed.
3
Don't shake it
Shaking can damage the peptide and cause foaming. Gentle swirling only.
4
Check it's fully clear
Only use it once it is fully clear with no particles. Label the vial with the date and 3.33 mg/mL, then store it in the fridge at 2–8 °C.

Full illustrated reconstitution guide

03 · SUPPLIES NEEDED

What you'll need

Counts follow the dosage chart above at the highest amount in each step, using a 10 mg vial mixed with 3 mL.

Length of protocol

1Sermorelin 10 mg vial7 mg needed in total

291 mL insulin syringesU-100, 27–31G · one per draw + one per vial mixed

1BAC water 10 mL bottle3 mL used for mixing

58Alcohol swabsSkin + stopper, every time

Plus an approved sharps container. Syringes and swabs are sold by Australian pharmacies — Peak Peptides and Pure Performance supply the bacteriostatic water.

Sermorelin 10 mg vialPeak Peptides Australia

Peak Peptides Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPeak Peptides Australia

Pure Performance Australia Bacteriostatic water 10 mL

Bacteriostatic water 10 mLPure Performance Australia

04 · CALCULATOR

Check a different amount

Enter your vial, water and dose to see the matching syringe units.

Concentration Calculator

Labelled Vial Amount (mg)

Verified Final Liquid Volume3 mL

Amount to Evaluate (not a recommendation)

mcg

Concentration

3.33 mg/mL

(3333 mcg/mL)

Calculated Liquid Volume

0.060 mL

Equivalent U-100 volume scale position

6markings (not biological IU)

Illustration of a U-100 volume scale, not preparation or injection guidance

05 · PROTOCOL DETAILS

About Sermorelin

Protocol overview

Starting dose: 200 mcg once nightly for weeks 1–2
How it increases: 300 mcg in weeks 3–4, 400 mcg in weeks 5–6, then 500 mcg in weeks 7–8
Frequency: Once nightly
When to take it: At bedtime, as stated by the attributed reference chart
Chart length: 8 weeks in the attributed reference chart
Common mix: 3 mL bacteriostatic water → 3.33 mg/mL

Storage

Before mixing: Keep the unopened vial refrigerated at 2–8 °C and away from light.
After mixing: Keep upright in the fridge at 2–8 °C, away from light, and use within about 28 days.
Never: Do not freeze the mixed vial or shake it vigorously.

How it works

Sermorelin is a synthetic, amidated 29-amino-acid fragment of growth hormone-releasing hormone, also called GHRH(1-29). It acts upstream of growth hormone at the anterior pituitary rather than supplying human growth hormone itself.

In a controlled physiology experiment, a 50 mcg intravenous dose of GRF(1-29) produced a significant growth-hormone rise in five healthy volunteers, with the peak at 15–30 minutes. Endocrine responses do not establish a clinical benefit for the reference-site schedule.

Illustrated mechanism explainer

What the research shows

A multicentre open-label study gave 30 mcg/kg/day subcutaneous GHRH(1-29) at bedtime to 110 prepubertal children with growth-hormone deficiency for up to 1 year; 86 were included in the efficacy analysis.
In that paediatric study, mean height velocity rose from 4.1 cm/year at baseline to 8.0 cm/year at 6 months and 7.2 cm/year at 12 months. This is weight-based paediatric evidence, not an adult 200–500 mcg protocol.
In five healthy volunteers, 50 mcg intravenous GRF(1-29) produced a significant growth-hormone increase versus placebo, peaking 15–30 minutes after dosing.
Historical Geref products were FDA-approved for selected paediatric growth-hormone-deficiency use in 1997, then discontinued and their approvals withdrawn effective 18 June 2009; FDA found they were not withdrawn for safety or effectiveness reasons.
FDA identifies no growth-hormone secretagogue approved to treat adult- or childhood-onset growth hormone deficiency in adults.

Reported side effects

The historical Sermorelin review reported transient facial flushing and injection-site pain as the most commonly reported adverse events with intravenous single doses and repeated once-daily subcutaneous doses.
In the 110-child study, investigators reported no adverse changes in general biochemical or hormonal analyses, no change in fasting glucose and no excessive insulin-like growth factor-1 generation over the reported follow-up.
These historical findings do not establish the quality, sterility or safety of a current research product, and adult evidence for the charted amounts is limited.

Important notes

The current 10 mg research listing is not a TGA- or FDA-approved medicine. Historical FDA approval of Geref does not approve this product or provide an adult protocol.
Prohibited in sport at all times: WADA lists Sermorelin under S2.2.4, growth hormone-releasing factors.
The directly relevant human treatment evidence was weight-based and in children with diagnosed growth-hormone deficiency, not an adult 200–500 mcg schedule.
The TGA warns that unapproved peptide products may not meet quality, safety or efficacy standards and may be incorrectly labelled.
Never use a mixed vial that is cloudy or has particles in it.

Injection basics

Use a new sterile 1 mL (100-unit) U-100 insulin syringe, 27–31G, every time — never reuse or share.
Wipe the vial stopper and the skin with an alcohol swab and let both dry first.
Inject under the skin (subcutaneously) of the abdomen, thigh or upper arm, and rotate sites.
Put used syringes straight into an approved sharps container — the NDSS lists Australian disposal options.

Guidance: World Health Organization · National Diabetes Services Scheme (NDSS)

06 · QUICK ANSWERS